SEC operates also within a preparative way by trapping the smaller molecules from the pores of a particles. The larger sized molecules merely go from the pores as These are way too big to enter the pores. Much larger molecules consequently stream in the column more quickly than more compact molecules: that may be, the smaller the molecule, the for
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The alter in eluent detected by a detector is in the shape of the electronic signal, and thus it remains to be not visible to our eyes.Where N is the quantity of theoretical plates, L is the length from the column, and H is the height comparable to a theoretical plate. The plate top is provided with the variance (standard deviation squared) of the
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